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Registros recuperados: 7
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Behavioural and biochemical responses of two marine invertebrates Scrobicularia plana and Hediste diversicolor to copper oxide nanoparticles ArchiMer
Buffet, Pierre-emmanuel; Tankoua, Olivia Fossi; Pan, Jin-fen; Berhanu, Deborah; Herrenknecht, Christine; Poirier, Laurence; Amiard-triquet, Claude; Amiard, Jean-claude; Berard, Jean-baptiste; Risso, Christine; Guibbolini, Marielle; Romeo, Michele; Reip, Paul; Valsami-jones, Eugenia; Mouneyrac, Catherine.
Engineered nano-sized Cu oxide particles are extensively used in diverse applications. Because aquatic environments are the ultimate "sink" for all contaminants, it is expected that nanoparticles (NP) will follow the same fate. In this study, two marine invertebrates Scrobicularia plana and Hediste diversicolor were chosen as ecotoxicological models. The aim was to evaluate behavioural (burrowing kinetics, feeding rate) and biochemical (biomarkers) responses of S. plana and H. diversicolor exposed in the laboratory to Cu (10 mu g L(-1)) added in natural seawater either in the form of engineered nanoparticles (NPs) of CuO or as dissolved Cu in 2% HNO(3). Exposure was characterized by considering (i) the physico-chemical fate of NP (ii) the fraction of...
Tipo: Text Palavras-chave: Copper oxide nanoparticles; Behaviour; Biomarkers; Oxidative stress; Scrobicularia plana; Hediste diversicolor.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15140/15856.pdf
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Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry ArchiMer
Poirier, Laurence; Amiard, Jean-claude; Mondeguer, Florence; Quiniou, Francoise; Ruiz, Nicolas; Pouchus, Yves Francois; Montagu, Monique.
Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found.
Tipo: Text Palavras-chave: Matched calibration; Matrix; Electrospray ionization ion trap mass spectrometry ESI IT MS; Marine fungal contamination; Trichoderma sp.; Fungal peptide metabolites.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2990.pdf
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Ecological and biological factors controlling the concentrations of trace elements (As, Cd, Cu, Hg, Se, Zn) in delphinids Globicephala melas from the North Atlantic Ocean ArchiMer
Caurant, Florence; Amiard, Jean-claude; Amiard-triquet, Claude; Sauriau, Pierre-guy.
Trace elements (As, Cd, Cu, Hg, Se and Zn) were determined in liver and kidney of pilot whales Globicephala melas Traill, 1809 collected from 7 schools caught at different seasons around the Faroe Islands. These and other biological data at our disposal enabled us to confirm and to define more accurately the relations shown previously between age, sex and trace element concentrations in marine mammals. The most striking features were: (1) the elevated levels of Cd and Hg in pilot whales compared to other marine mammals and to minimum adverse-effect levels established for humans; (2) the biocumulative behaviour of Cd and Hg; (3) the high correlations between Hg and Se, predominantly influenced by age; and (4) the high correlations between Cd and Zn, the...
Tipo: Text Palavras-chave: Marine mammals; Heavy metals; Interactions.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00196/30766/29149.pdf
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Etude in situ et expérimentale de perturbations du système endocrinien chez un mollusque, un crustacé et deux poissons marins et / ou estuariens sous l'influence de quelques métaux (Cd, Pb, Cu, Zn) ArchiMer
Andersen, A.; Amiard, Jean-claude; Amiard-triquet, Claude; Ferrand, R.; L'Hermite, A.; Metayer, C..
Les tests écotoxicologiques qualifiés de sub-léthaux se rapportant aux perturbations des grandes fonctions physiologiques, mettent bien souvent en oeuvre au laboratoire des concentrations de polluants infiniment supérieures à celles susceptibles d'être rencontrées dans les milieux marins même les plus pollués, à l’exception de déversements accidentels massifs. Nous avons pensé pouvoir nous rapprocher davantage des conditions naturelles du milieu en étudiant des altérations des mécanismes les plus intimes de la vie animale tels que des perturbations du système endocrinien chez des organismes soumis- à une pollution métallique.
Tipo: Text
Ano: 1984 URL: http://archimer.ifremer.fr/doc/00151/26196/24215.pdf
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Influence of ploidy and metal-metal interactions on the accumulation of Ag, Cd, and Cu in oysters Crassostrea gigas thunberg ArchiMer
Amiard, Jean-claude; Perrein Ettajani, H; Gerard, Andre; Baud, Jean-pierre; Amiard Triquet, C.
The present study was designed to compare the response to contaminants in diploid with triploid specimens of the oyster Crassostrea gigas. The reproduction investment in bivalve molluscs has priority on somatic growth. Thus, genetic sterilization by triploidy induction enables the energy flux to be directed toward somatic growth and glucide storage. Bioaccumulation was examined for Ag (10 mug/L), Cd (10 mug/L), and Cu (30 mug/L) to determine if the response to metals follows similar patterns in diploid (2n) and triploid (3n) groups. The effect of ploidy was also evaluated as a function of dry weight of soft tissue and condition index. Moreover, the reciprocal influence of these metals on their incorporation was studied. The results showed that the major...
Tipo: Text Palavras-chave: Oyster culture; Metal bioaccaumulation; Polyploidy.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-791.pdf
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Shellfish and Residual Chemical Contaminants: Hazards, Monitoring, and Health Risk Assessment Along French Coasts ArchiMer
Gueguen, Marielle; Amiard, Jean-claude; Arnich, Nathalie; Badot, Pierre-marie; Claisse, Didier; Guerin, Thierry; Vernoux, Jean-paul.
Shellfish farming is a common industry along European coasts. According to the 2005–2006 data from the French National Shellfish Farming Committee (CNC – Comité National de la Conchyliculture 2010; see Table 1 for a list of acronyms and abbreviations used in this chapter), Spain is the largest shellfish producer in Europe (∼270,000 t) and France ranks second, producing 200,000 t of shellfish annually. France is the leading European oyster producer, with an annual output of 130,000 t of Crassostrea gigas, and ranks fourth in the world after China, Japan, and Korea. The top three European mussel (Mytilus edulis and Mytilus galloprovincialis) producers are Spain (260,000 t), Denmark (80,000 t), and France (65,000 t). For other shellfish, the French annual...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00039/14995/12622.pdf
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Toxicity assessment of peptaibols and contaminated sediments on Crassostrea gigas embryos ArchiMer
Poirier, Laurence; Quiniou, Francoise; Ruiz, Nicolas; Montagu, Monique; Amiard, Jean-claude; Pouchus, Yves François.
Peptaibols are known membrane-modifying peptides that were recently detected in marine sediments and mussels collected from a shellfish farming area (Fier d'Ars, Atlantic coast, France). In this investigation, embryotoxicity bioassays with oysters (Crassostrea gigas) were performed to assess acute toxicity of alamethicin and different groups of peptaibols produced by a Trichoderma longibrachiatum strain isolated from marine environment. C gigas embryos appeared very sensitive to all the metabolites examined with higher toxic effects for long-sequence peptides (EC50 ranging from 10 to 64nM). D-shaped larvae with mantle abnormality were particularly noticed when peptaibol concentrations increased. Disturbances of embryogenesis were also observed following...
Tipo: Text Palavras-chave: Risk assessment; Embryotoxicity; Bivalve bioassay; Mycotoxins; Marine fungi.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2680.pdf
Registros recuperados: 7
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